Fig. 3

Zmp-2 RNAi decreases vit-6 expression and DHE uptake. Real-time quantitative PCR (a) and Western blot analysis with β-actin as a loading control (b) show the significantly decreased vit-6 expression at the mRNA and protein level, respectively, in nematodes treated with zmp-2 RNAi. Uptake of dehydroergosterol was measured according to its fluorescent properties using fluorescence microscopy (c) and quantified using ImageJ (c, right lower panel). **p < 0.01; ***p < 0.001 versus the N2 vector control; ^## p < 0.01 versus zmp-2 RNAi-treated nematodes