Table 1 Oligonucleotide primers used for quantitative real-time PCR
Genea | Sequence (5′–3′) | F/Rb | Template |
|---|---|---|---|
GAPDH | TCCACTGGCGTCTTCACC | F | cDNA |
GGCAGAGATGATGACCCTTT | R | ||
GAPDH | ATGACAAGCATGAGGCAGAG | F | gDNA |
CAACCAGGACCGTTAACCCTTTCT | R | ||
IL-1α | ACAAAAGGCGAAGAAGACTGA | F | cDNA |
GGAACTTTGGCCATCTTGAC | R | ||
IL-1α (−319 to −56) | CCAACTCACACAAGCTGCTTT | F | gDNA |
GGTGGTAGAACACCAGACTCTT | R | ||
IL-1α (+128 to +416) | CTTCTTCTACAGAAGACACACCTT | F | gDNA |
CTGAAGAGGGAAGTTTGCTTGATT | R | ||
IL-1β | CTGTCCTGCGTGTTGAAAGA | F | cDNA |
TTGGGTAATTTTTGGGATCTACA | R | ||
IL-1β (−235 to −67) | ATATTTGCATGGTGATACATTTGC | F | gDNA |
CTCTGTTGAATACCTGATTTCACAA | R | ||
IL-1β (+127 to +338) | GCCTCTTTGTGTGTATGCATATT | F | gDNA |
GAGAGCTGGAGCAGAGGCTT | R | ||
IL-6 | TCCAGAACAGATTTGAGAGTAGTG | F | cDNA |
GCATTTGTGGTTGGGTCAGG | R | ||
IL-6 (−279 to −93) | CTTCGTGCATGACTTCAGCTTT | F | gDNA |
GATTGTGCAATGTGACGTCCTTT | R | ||
IL-6 (+132 to +398) | CACAAGTAAGTGCAGGAAATCCTT | F | gDNA |
CGGCTACATCTTTGGAATCTT | R | ||
LSD1 | ACCACAACAGACCCAGAAGG | F | cDNA |
GGTGCTTCTAATTGTTGGAGAG | R | ||
TNF-α | GCTCTTCTGCCTGCTGCACTT | F | cDNA |
GATGGCACCACCAGCTGGTT | R | ||
TNF-α (−399 to −192) | CCTGCATCCTGTCTGGAAGTT | F | gDNA |
GGGACACACAAGCATCAAGGAT | R | ||
TNF-α (+103 to +374) | GCTGCCAGGCAGGTTCTCTT | F | gDNA |
GGCCAGGCACTCACCTCTT | R |