Fig. 1
Time-course of E2 and Nar effect on differentiation markers in L6 cells. L6 cells were grown in the differentiation medium containing 2 % serum. Western blot analysis of vehicle (DMSO:PBS 0.1:1), E2 (10−8 M), and Nar (10−6 M) treatment on myogenin (a), Fetal MHC (b) levels and related densitometric analyses (c). d Vehicle, E2 and Nar effect on GLUT-4 plasma membrane translocation ( d) and related densitometric analysis. The amount of proteins was normalized by comparison with tubulin (a and b) or cav-1 (d) levels. Data are the mean of five different experiments ± SD. * P < 0.001 was calculated with ANOVA followed by Tukey–Kramer post test with respect to the vehicle-treated samples