Fig. 4
Effect of E2 and Nar on slow and fast MHC level in satellite cells and in C2C12 myoblasts. Typical Western blot and densitometric analyses of slow and fast MHC levels in satellite cells (a) and in C2C12 myoblasts maintained in differentiation medium for 48 h in the presence of either vehicle (DMSO:PBS 0.1:1) or E2 (10−8 M) or Nar (10−6 M). The amount of proteins was normalized by comparison with tubulin levels. Data represent the mean ± SD of five experiments. P < 0.001 was calculated with ANOVA followed by Tukey–Kramer post test with respect to vehicle-treated samples (asterisk) or to E2-treated samples (open circle)