Fig. 6
Nar effect on AKT activation and GLUT-4 plasma membrane translocation. Typical Western blot and densitometric analyses of AKT phosphorylation (a, b, and c) and GLUT-4 plasma membrane translocation (d and e) in L6 cells maintained in differentiation medium for 48 h and stimulated for 1 h (a, b, and c) or 30 min (d and e) with either vehicle (DMSO:PBS 0.1:1) or E2 (10−8M) or IGF (100 ng/ml) and/or Nar (10−6 M). When indicated cells have been pre-treated (30 min) with the AKT inhibitor (10−6M). Data represent the mean ± SD of five experiments. P < 0.001 was calculated with ANOVA followed by Tukey–Kramer post test with respect to vehicle-treated samples (asterisk) or to E2-treated samples (open circle)