Fig. 7
Nar effect on p38 activation and myogenin and fetal MHC level. Typical Western blot and densitometric analyses of p 38 phosphorylation (a), myogenin, and fetal MHC levels (b, c, and d) in L6 cells maintained in differentiation medium for 48 h and stimulated for 1 h (a) or 48 h (b, c, and d) with either vehicle (DMSO:PBS 0.1:1) or E2 (10−8 M) or IGF(100 ng/ml) and/or Nar (10−6 M). When indicated cells have been pre-treated (20 min) with p38 inhibitor (SB, 10−6 M, b) or with the AKT inhibitor (10−6 M, c). Data represent the mean ± SD of four experiments. P < 0.001 was calculated with ANOVA followed by Tukey–Kramer post test with respect to vehicle-treated samples (asterisk) or to E2-treated samples (open circle)