Fig. 8
Nar and E2 effect on H2O2-induced ROS production in L6 myoblasts. L6 cells, maintained in differentiation medium for 48 h, were pre-treated, as indicated, with either vehicle (DMSO:PBS 0.1:1) or E2 (10−8 M) or Nar (10−6 M) or estrogen receptor inhibitor (ICI 182,780, ICI, 10−6 M) or the ERα agonist (PPT, 10−8 M) or the ERβ agonist (DPN, 10−8 M) or the ERβ antagonist THC (10−6 M) before exposition to H2O2 (2 × 10−4 M) for 15 min. In a typical original output (arbitrary units) of the registrations captured by the spectrofluorimeter during 15 min substance administration are reported. In b and c are reported data expressed as % of variation between H2O2-stimulated fluorescence versus basal fluorescence for each stimulation. Data are the mean ± SD of three experiments. P < 0.001 was calculated with ANOVA followed by Tukey–Kramer post test with respect to vehicle-treated samples (asterisk) or to E2-treated samples (open circle) or to DPN-treated samples (plus sign)or to Nar-treated samples (hash)