Fig. 3

Multi-level network model of eWAT adaptation to 5 days of HFD. The three-layered network model comprising (1) biological processes, (2) transcription regulators (TFs) and (3) physiological parameters associated with eWAT gene expression after 5 days of HFD feeding. The processes layer includes differentially enriched biological processes as described in Fig. 1. The regulatory network layer includes TFs whose targets are enriched among the differentially expressed genes (HFD vs. LFD after 5 days of HFD feeding). TFs with highly overlapping target gene sets are clustered into single nodes. The physiological network layer includes parameters significantly correlated with eWAT expression data. The connections between the three layers are based on the overlap between underlying gene sets. The width of edges is based on the overlap between underlying gene sets. The colour coding of nodes is as described in Fig. 1, where for TFs, the direction of the expression of their targets is represented (red—activation, blue—repression)