Fig. 5
Effects of n-3 polyunsaturated fatty acid and 17β-estradiol-3-benzoate (E2) on the expressions of estrogen receptor (ER) in skeletal muscle. Cytosol fraction of ERα (a) and ERβ (b) and nuclear fraction of ERα (c) and ERβ (d). Values are expressed as mean ± SEM (n = 8); 0, 1, and 2 % n-3, 0, 1, and 2 % EPA + DHA diets with corn oil injection; 0, 1, and 2 % n-3 + E2, 0, 1, and 2 % EPA + DHA diets with E2 injection. Asterisk values are significantly different (P < 0.05) between corn oil and E2 injection for diets containing the same amount of n-3 PUFA. Dagger values are significantly different (P < 0.05) among 0, 1, and 2 % n-3 within the corn oil- or E2-injected groups