Fig. 1

Curcumin increases the SMN protein and the proportion of SMN2_E7+ transcript in fibroblast cells form a patient with SMA type II. a A Western blot of SMN protein levels in the cells treated with DMSO or curcumin for 48 h, and b time course of the changes (mean ± SD, n = 3, relative to β-ACTIN) as in a. c An agarose gel of RT-PCR products of the SMN2 variants (E7+ and E7−) in the cytoplasm, from the cells treated with curcumin in a time course, and d time course of the exon 7-containing SMN2 transcript changes (SMN2_E7+ mol%) calculated as the molar intensity of the 364-bp product (SMN2_E7+) relative to the total of the 364- and 310-bp (SMN2_E7−) products (mean ± SD, n = 3) in each lane of the gel as in c. **p < 0.01, ***p < 0.001, compared to time 0