Fig. 6
From: Multiple effects of curcumin on promoting expression of the exon 7-containing SMN2 transcript
SRSF1 knockdown reduces the inclusion of SMN2 exon 7. a Western blots of SRSF1 or hnRNP A1 from the BJ301J cells 7 days after transduction of lentiviral particles containing interfering shRNA. β-ACTIN protein loading control. b Upper panel Agarose gels of RT-PCR products of the SMN2 variants in the cytoplasm of the BJ301 cells knocked down of indicated factors. Cells were treated for 24 h with DMSO or curcumin 7 days after transduction. GAPDH RNA loading control. Lower panel A bar graph of the molar percentages of exon 7-containing SMN2 transcript (SMN2_E7+), calculated as the molar intensity of the 364-bp product (SMN2_E7+) relative to the total of the 364- and 310-bp (SMN2_E7−) products (mean ± SD, n = 3) in each lane of the gel shown in the upper panel