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Table 1 Primers used for RT-PCR and real-time PCR

From: Altered expression of two zinc transporters, SLC30A5 and SLC30A6, underlies a mammary gland disorder of reduced zinc secretion into milk

Forward primer

Reverse primer

PCR product length (bp)

ZnT1 -RealF1

CTGGTGAACGCCATCTTCCT

ZnT1 -RealR1

CAATCTCGTGCGGCTCGAT

183

ZnT2 -RealF2

TGTGATCCTGGTGTTGATGGA

ZnT2 -RealR2

CAGGCTGTGCAGGGCTTCT

103

ZnT3 -RealF1

CACCCTCCGAGACGTTCTTC

ZnT3 -RealR1

GGCACCGACAACAGCGTAT

90

ZnT4 -RealF2

AACCAGTCTGGTCACCGTCA

ZnT4 -RealR2

CTATCCTGCCCATGGTTACG

93

ZnT5 -RealF1

CCAGCGCTCGATTAACAAAATA

ZnT5 -RealR1

TGTGAACAGCTTTTAGGAGATCA

134

ZnT6 -RealF2

TCCTTTTTTGGCAAGTTGTTACG

ZnT6 -RealR2

AAGCAGGAAGCCAGTACATATCAAG

111

ZnT7 -RealF1

TTGCCCCTGTCCATCAAAG

ZnT7 -RealF2

AGACCTAAACCAGCCCGAGATC

81

ZnT8 -RealF1

ACCATGGTCATAGCTTACGA

ZnT8 -RealF2

CGAATCAGTACCGGCAATTTA

157

ZnT9 -RealF1

AACGGCATTAGCATCGTATG

ZnT9 -RealF2

GTAACGTGAAACTTGGATCGAT

114

ZnT10 -RealF1

AGCTTTCGCAATGGCTTAGG

ZnT10 -RealF2

GTACCGTAACTGGTTAATATCG

95

βAC - RealF

GACAGGATGCAGAACGAGAT

βAC - RealR

TGATCCACATCTGCTGGAAGGT

138

GAPDH - RealF

CCACCCATGGCAAATTCC

GAPDH - RealR

TGGGATTTCCATTGATGACAA

145

ZnT5 -A

GCAGCGGCGAGACATGAGGAG

ZnT5 -B

CACTACCACTCCTCCAGACAG

1113

ZnT5 -C

TCTCTCATTATGCCTTTTGC

ZnT5 -D

GCATCTTTAATCAGTGGAACA

1125

ZnT5 -E

CAGCATTGGTGTGATCGTATC

ZnT5 -F

AAGATTCCTTGATCCAGTAGT

629

ZnT6 -A

AGAACGGCTTCCGGCGGG

ZnT6 -B

GAGGCATTGGGATTACGTGA

1131

ZnT6 - C

AAGTCTTACTCCAGACAACA

ZnT6 -D

AATGTGAACAAGACTACTAT

758

ZnT2 - 1F

CGTCCTCACTCAGCAACACC

ZnT2 - 1R

TCTTCGTTCCCTCACCTCAC

658

ZnT2 - 2F

AGGACTCCCATTCCCCTATC

ZnT2 - 2R

AGGGAGAATAACGTCACCCATGT

582

ZnT2 - 3F

ATATTGGTGGCCCATTTCAC

ZnT2 - 3R

ACAGAGGCCATGGTTGACAT

503

ZnT2 - 4F

ATATGAGGGGTGGGGTAAGG

ZnT2 - 4R

ACAGCTCCCAGTGTTCTTGG

527

ZnT2 - 5F

GCCCTATCTCTCATGGCTGT

ZnT2 - 5R

CCACCTACCCTTCAGGTTGT

533

ZnT2 - 6F

TTCTGAACTGTGGTCTGTCCTT

ZnT2 - 6R

GAACTGCCAGACCTGAAAGC

501

ZnT2-7F

TCAGTGTTAAGAGTGGAGAGGAA

ZnT2 - 7R

AACCCCAGCCTCAGTTTCTT

530

ZnT2 - 8F

TACCTGGCCAGAGGAATGGAA

ZnT2 - 8R

GAAAGGGAACATTTGGCTCA

848

ZnT2 - 9F

TATGAATCTGAGCCCCTCCA

ZnT2 - 9R

CAAGTGACCAAACCCACCTC

912

ZnT2 - C5F

ACTGGTGTACCTGGCTGTGG

ZnT2 - C5R

CCGAGTAGTCCTCGATCTGG

210

ZnT5 - P1F

TCAGGCTCCTCTCATTCTCC

ZnT5 - P1R

TGTTTCTTACAGCCCCGAAC

1256

ZnT5 - P2F

GGCAAGTCCATAAAGTGAAGC

ZnT5 - P2R

CTGAGGAGGAGAATGGCTTG

1189

ZnT5 - P3F

CAAGCCATTCTCCTCCTCAG

ZnT5 - P3R

GACCAAAATTTGCCTCCTCA

1098

ZnT5 - P4F

GAGACAGGGTTTGCCATGT

ZnT5 - P4R

CAAGCAGAACACTCGCCTTT

1045

ZnT5 - P5F

TGGGAATACAGCCCAGTAGG

ZnT5 - P5R

GACCAGATTAATGCCAGAGC

645

ZnT5 - P6F

CCATACTGCCCAGACCCTTA

ZnT5 - P6R

ATGGCGAAACCCTGTCTCTA

432

ZnT5 - P7F

AGCCACAAAAGCAGTCACCT

ZnT5 - P7R

AAAATTTGCCTCCTCAGCAA

684

ZnT5 - P8F

AGGAGAATCGCTTGAACCTG

ZnT5 - P8R

GGAGAAAGTGCATCCTGAGC

750

ZnT5 - P9F

TCCCAAATTTCCCAGAGTA

ZnT5 - P9R

GTGGGGAGCTTACAAAATGG

243

ZnT5 - P10F

CCATACTGCCCAGACCCTTA

ZnT5 - P10R

GAGACCAGCCTGACCAACAT

189

ZnT5 - P11F

CCATCTGGACCCATCAGTCT

ZnT5 - P11R

CCTGGCCCTGAATTCTTTCT

530

ZnT5 - C1F

CTGATGACGTGGCTTGGC

ZnT5 - C1R

CGAAAAGTCCCACAGCCTTC

120

ZnT6 - P1F

AAGGGTGTCAGCAGAGATGG

ZnT6 - P1R

GGGACTGTCATGATGGGATT

1170

ZnT6 - P2F

CACCTTCTCACCGTGTCCTT

ZnT6 - P2R

AGGCAGGAGAATTGCTTGAA

1037

ZnT6 - P3F

TCTCACCCAGGATTCATCTC

ZnT6 - P3R

TGTAGCACGTGTGTGTTTGTCAGA

1168

ZnT5 - P4F

TTAAGCATTTGGGATGAAGGA

ZnT5 - P4R

GTGCTCGAGTTTTCCCAGAA

1076

ZnT5 - P5F

GGAGGCCAAGGCAGGTAG

ZnT5 - P5R

GAATTGCTTGAACCCAGGAG

985

ZnT5 - P6F

TTTCTTCCTGGCTTGCAGAT

ZnT5 - P6R

GGTTTCACCATGTTGTGCAG

756

ZnT5 - P7F

TGGGCAGCTTAAATAACAGAAA

ZnT5 - P7R

GTGATTCAGCCTTTGGGAAG

468

ZnT5 - P8F

TCAGCAACCCTAATCCAACC

ZnT5 - P8R

TGTAGCACGTGTGTTTGTCA

496

ZnT5 - P9F

CGAAAAACTCCTCGTTGCTG

ZnT5 - P9R

ACTCCAATGACCCCTCCTCT

278

ZnT5 - P10F

GGAGGCCAAGGCAGGTAG

ZnT5 - P10R

ATGGCAAAACCCCGTCTC

543

ZnT5 - P11F

ACCCATCAGAAGAAATAAACCA

ZnT5 - P11R

ATTTGAGGCCAGGCACAGT

272

ZnT6 - C1F

TTGACTCCTTGGCTTCCAAT

ZnT6 - C1R

AAAGGAGAGAAACTTTAACAAAAATC

103

ZnT2IE - 1F

CGTCCTCACTCAGCAACACC

ZnT2IE - 1R

TCTTCGTTCCCTCACCTCAC

98

ZnT2IE - 2F

AGGACTCCCATTCCCCTATC

ZnT2IE - 2R

AGGAGAATAACGTCACCCATGT

128

ZnT2IE - 3F

ATATTGGTGGCCCATTTCAC

ZnT2IE - 3R

ACAGAGGCCATGGTTGACAT

136

ZnT2IE - 4F

ATATGAGGGGTGGGGTAAGG

ZnT2IE - 4R

ACAGCTCCCAGTGTTCTTGG

189

ZnT2IE - 5F

GCCCTATCTCTCATGGCTGT

ZnT2IE - 5R

CCACCTACCCTTCAGGTTGT

97

ZnT2IE - 6F

TTCTGAACTGTGGTCTGTCCTT

ZnT2IE - 6R

GAACTGCCACACCTGAAGC

84

ZnT2IE - 7F

TCAGTGTTAAGAGTGGAGAGGAA

ZnT2IE - 7R

AACCCCAGCCTCAGTTTCTT

95

ZnT2IE - 8F

TACCTGGCAGAGGAATGGAA

ZnT2IE - 8R

GAAAGGGAACATTTGGCTCA

178

ZnT2IE - 9F

TATGAATCTGAGCCCCTCCA

ZnT2IE - 9R

CAAGTGACCAAACCCACCTC

153

  1. Primer names contain the name of the gene, and following abbreviations: Real real-time PCR primer, AF primers for coding region sequencing, P promoter region primers, IE intron–exon primers
  2. Primers to ZnT4 were designed to GenBank sequence Accession No AF025409
  3. Primers to ZnT1 were designed to GenBank sequence Accession No AF323590
  4. Primers to ZnT2 were designed to GenBank sequence Accession No NM_032513
  5. Primers to ZnT3 were designed to GenBank sequence Accession No U76010
  6. Primers to ZnT5 were designed to GenBank sequence Accession No AF461760
  7. Primers to ZnT6 were designed to GenBank sequence Accession No XM_059432
  8. Primers to ZnT7 were designed to GenBank sequence Accession No AY094606
  9. Primers to ZnT8 were designed to GenBank sequence Accession No AY117411
  10. Primers to ZnT9 were designed to GenBank sequence Accession No BC016949
  11. Primers to ZnT10 were designed to GenBank sequence Accession No AY117411
  12. Primers to β-actin were designed to GenBank sequence Accession No E00829