Fig. 3

Effect of MLE on the expression of genes involved in hepatic lipogenesis, lipolysis, and fibrosis in high-fat diet-induced obese mice. Relative mRNA expression related to a lipogenesis, b thermogenesis, and c fibrosis. d Protein levels of SREBP1c, C/EBPα, PPARα, FAS, LPL, and α-SMA. Both mRNA and protein samples were pooled from 6 mice per group. A representative image of repeated experiments is shown in the left panel. The bands show the intensity of the bands that were densitometrically measured and normalized to the band levels of GAPDH for RT-PCR or lamin B1 (nucleus) and β-actin (cytosol) for western blot, respectively. Values are mean ± SEM and tested by ANOVA with Duncan’s test for multiple comparisons. Values with the same superscript letter are not significantly different (P < 0.05). CON, normal control diet-fed mice; HF, high-fat diet-fed control; L, low-dose mulberry leaf extract (133 mg/kg)-treated high-fat diet-fed mice; H, high-dose mulberry leaf extract (666 mg/kg)-treated high-fat diet-fed mice