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Fig. 3 | Genes & Nutrition

Fig. 3

From: Characterization of the hypothalamic transcriptome in response to food deprivation reveals global changes in long noncoding RNA, and cell cycle response genes

Fig. 3

microRNA array plots and QPCR analysis. a log2 scatter plot of hypothalamus microRNA array data from Affymetrix array. Data were filtered using a P value <0.05 and 1.3-fold change. b Volcano plot showing P value versus log2 expression level for data from Affymetrix array. Lines indicate where significance cutoff values were made. c microRNA QPCR analysis in hypothalamic tissue of ad lib versus food-deprived mice. microRNAs were selected using the cutoff criteria, and expression level tested in N = 4–6 new samples for each group. All microRNA levels are reported relative to levels in ad lib fed animals and normalized to the sno-202 microRNA. d microRNA expression in skeletal muscle versus hypothalamus. *P ≤ 0.05; **P ≤ 0.01

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