Fig. 2
From: Effect of a diet enriched with omega-6 and omega-3 fatty acids on the pig liver transcriptome
Validation of differentially expressed genes (DEGs) by qRT-PCR. a comparison of the RNA-Seq (6 animals per group) and qRT-PCR (15 animals per group), Log2-fold of change between experimental and control groups, b individual variability of validated differentially expressed genes in qRT-PCR. For qRT-PCR, fold change was assessed on the basis of mean values of gene expression for 2 dietary groups with 15 individual samples per group. All Ct values were normalized to the topoisomerase (DNA) II beta (TOP2B) gene. Error bars represent standard error of variability among individual samples. *p-value <0,05; **p-value <0,01; ***p-value <0,001, ns not significant