Effect of a diet enriched with omega-6 and omega-3 fatty acids on the pig liver transcriptome

Genes & Nutrition

Table 2 Oligonucleotide primer sequences used for quantitative real-time PCR experiments

Gene	Sequences (5′–3′)	Amplicon size (bp)	Annealing temperature (°C)	GenBank accession no.
DGAT2	For. GACCCTCATAGCTGCCTACTC	136	59	NM_001160080.1
DGAT2	Rev. CAGCACGGAGATGACCTGTA	136	59	NM_001160080.1
ELOVL6	For. GAACACGTAGCGACTCCGAA	178	59	XM_003357048.2
ELOVL6	Rev. ATGCCGACCGCCAAAGATAA	178	59	XM_003357048.2
EXTL1	For. TGGGATGGGCACTGTGAGC	78	59	XM_003356212.1
EXTL1	Rev. GCAGAAGGTGGCATTGGGTA	78	59	XM_003356212.1
MMP2	For. CGGACAAAGAGTTGGCTGTG	158	59	NM_214192
MMP2	Rev. CATGGTCTCGATGGTGCTCT	158	59	NM_214192
COL1A1	For. TTCAGCTTTGTGGACCTCCG	136	59	XM_005668927
COL1A1	Rev. CGTTCTGTACGCAGGTGACT	136	59	XM_005668927
ACSL1	For. TCAGAAGGTTGCCAGTGAAG	115	59	NM_001167629
ACSL1	Rev. CTGGAGGAGAGGATCAGAGAATA	115	59	NM_001167629
APOA4	For. CAGCAGCTCAACACTCTCTT	142	59	NM_214388
APOA4	Rev. GAATCTCCTCCTTCAGCTTCTC	142	59	NM_214388
APOA5	For. GGAAGAGAAGGGAAGAAGGAAG	83	59	NM_001159308
APOA5	Rev. CATCGGCCAACAGGGATATT	83	59	NM_001159308
FADS1	For. GGGCCTTGTGAGGAAGTATATG	104	59	NM_001113041
FADS1	Rev. GGAACTCATCTGTCAGCTCTTT	104	59	NM_001113041
FADS2	For. CCTTACAACCACCAGCATGA	118	59	NM_001171750
FADS2	Rev. CCAAGTCCACCCAGTCTTTAC	118	59	NM_001171750
FASN	For. CTTGTCCTGGGAAGAGTGTAAG	83	59	NM_001099930
FASN	Rev. AGATGGTCACCGTGTCTTTG	83	59	NM_001099930
TOP2B	For. AACTGGATGATGCTAATGATGCT	137	59	NM_001258386.1
TOP2B	Rev. TGGAAAAACTCCGTATCTGTCTC	137	59	NM_001258386.1

ISSN: 1865-3499