Fig. 5
From: Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans
Choline abrogates the extension of the reproductive period and increased survival rate in the cold in the P0 and F1 generations, but not the increase in the life span of dietarily restricted worms. a Survival curves of ad libitum-fed (AL) and dietarily restricted (DR) young adult worms without (H2O vehicle control; AL, DR) or with choline (AL CH, DR CH) supplementation, following their transfer from 20 to 0 °C. The data were obtained in three independent experiments. b, c Survival curves of AL-fed L1 larvae (b) or AL-fed L4 larvae (c) obtained from either AL-fed or DR adult worms, without (H2O vehicle control; AL, DR) or with choline (AL CH, DR CH) supplementation, following their direct transfer from 20 to 0 °C. The data were obtained in three independent experiments. DR/AL→AL, DR/AL) of the P0 generation and AL of F1 larvae; DR CH →AL, DR with choline supplementation of the P0 generation and AL feeding of F1 larvae. d–f Time-course of the progeny production (d), length of the reproductive period (e), and lifetime progeny production rate (f) of ad libitum-fed (AL) and dietarily restricted (DR) worms, without (H2O vehicle control; AL, DR) or with choline (AL CH, DR CH) or methionine (AL M, DR M) supplementation. g Life span curves representing the typical results of two to four independent experiments using ad libitum-fed (AL) and dietarily restricted (DR) worms, without (H2O vehicle control; AL, DR) or with choline (AL CH, DR CH) or methionine (AL M, DR M) supplementation