Table 1 Primers used for real-time qPCR; full names of genes are available in Table 2
No. | Gene symbol | Forward primer | Reverse primer | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|---|---|
1. | bdnf | CCCCATGAAAGAAGCAAACG | TACAAGTCCGCGTCCTTACT | 60 | 213 |
2. | btg1 | GGCTCCATCTGTGTGCTGTA | GCCCACCCAAAGCAAAACTC | 60 | 227 |
3. | gja1 | TGCTGCGAACCTACATCATC | CGATGACGTTCAAGGCAAGA | 60 | 255 |
4. | igf1 | CAGAAGCAATGGGAAAAATCA | CGTGGGCTTGTTGAAATAAAA | 62 | 242 |
5. | itpr2 | CTGTGGGTATTCGGCCATTT | GCCACGATTTCCGACAAAAC | 60 | 143 |
6. | mllt3 | GGAACCGAAACCCATGTCAA | GGGCTTTTTGTCAGCAGAAC | 60 | 220 |
7. | mstn | GCCTGGAAACAGCTCCTAAC | GTCGTCGCGTGGTAATCATC | 60 | 151 |
8. | myf5 | GGAGACGCCTGAAGAAAGTC | CCGGCAGGCTGTAGTAATTC | 60 | 171 |
9. | myog | CTCGCTCACCTCCATTGTG | CAGTTGGGCATGGTTTCATC | 60 | 78 |
10. | tgfb2 | AGTACTACGCCAAGGAGGTT | TAGGCGGGATGGCATTTTCC | 60 | 72 |
11. | gapdh | GTTTGTGATGGGCGTGAACC | GTCTTCTGGGTGGCAGTGAT | 60 | 198 |