Fig. 5
From: Metallothioneins 1 and 2, but not 3, are regulated by nutritional status in rat white adipose tissue
The influence of fasting or fasting/refeeding on MT1 mRNA level in WAT (a), liver (b, c), MT1 and MT2 (MT1/2) protein level in WAT and liver of rats (d), zinc level (μg/mg protein) in epididymal WAT (e), ZnT1 mRNA level in epididymal WAT (f), and MTF1 mRNA level in epididymal WAT(g). MT1 mRNA levels relative to β-actin expression in WAT (retroperitoneal, epididymal, and inguinal) and liver of rats fed ad libitum (CN), fasted 48 h (F48), and fasted 48 h and refed 12 h (F48 + 12) are shown on the graphs (mean ± S.D., n = 10) *p < 0.05, **p < 0.01, ***p < 0.001; MT1 and MT2 protein level in WAT and liver of rats fed ad libitum (CN), fasted 48 h (F48), and fasted 48 h and refed 12 h (F48 + 12) protein levels were assessed by western blotting (two representative samples from each group are shown, 20 μg of protein per lane was loaded); ZnT1 and MTF1 mRNA levels relative to β-actin mRNA levels in WAT (epididymal) of rats fed ad libitum (CN), fasted 48 h (F48), and fasted 48 h and refed 12 h (F48 + 12) are shown on the graphs (mean ± S.D., n = 10)