Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice

Table 1 Proteins separated by 2-DE electrophoresis and identified by MS in liver tissue

Spot^a	Identification (Swiss-Prot)^b	Gene^c	Sequence^c coverage (%)	Spot^d MW (KDa)	Spot^e pI	Score^f	Swiss^g Prot access #
1	Annexin A5 (ANXA5)	Anxa5	72	35.8	4.8	631	P48036
2	Ketohexokinase (KHK)	Khk	61	32.8	5.8	475	P97328
3	Major urinary protein 6 (MUP6)	Mup6	52	20.7	4.7	284	P02762
4	Major urinary protein 2 (MUP2)	Mup2	73	20.7	4.9	227	P11589
5	Major urinary protein 2 (MUP2)	Mup2	51	20.7	4.9	96	P11589
6	Glutathione S-transferase P1 (GSTP1)	Gstp1	39	20.6	8.1	211	P19157
7	Bile salt sulfotransferase 2 (ST2A2)	Sult2a2	10	33.3	6.9	45	P50236
8	Alanine-TRNA ligase, cytoplasmic (SYAC)	Aars	1	106.9	5.4	34	Q8BGQ7
9	Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1)	Acox1	15	74.7	8.6	177	Q9R0H0

^a2-DE gel image spot number presented in Fig. 1
^bCommonly used protein name
^cGene symbol
^dPercentage of amino acid sequences for the identified protein
^eMW and pI = molecular weight and isoelectric point of the protein
^fScore = [−10 log (P)] and P is the absolute probability that the observed match between the experimental data and the database sequence is a random event, obtained with MASCOT for all proteins listed
^gSwiss-Prot primary protein accession number

ISSN: 1865-3499