Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice

Table 2 Proteins separated by 2-DE electrophoresis and identified by MS in adipose tissue

Spot^a	Identification (Swiss-Prot)^b	Gene^c	Sequence^c coverage (%)	Spot^d MW (KDa)	Spot^e pI	Score^f	Swiss^g Prot access #
1	Indolethylamine N-methyltransferase (INMT)	iNMT	40	29.5	6.0	282	P40936
2	Destrin (DEST)	DSTN	41	18.4	8.2	126	Q9R0P5
3	Pyridoxal kinase (PDXK)	Pdxk	26	35.0	5.9	116	Q8K183
4	Glutathione S transferase theta-1 (GSTT1)	GSTT1	32	27.2	7.2	181	P30711
5	Mitochondrial peptide methionine sulfoxide reductase (MSRA)	Msra	19	23.8	6.7	27	Q9D6Y7
6	Endoplasmic reticulum resistant protein 29 (ERP29)	Erp29	29	25.7	5.7	185	P57759
7	Delta-amino levulinic acid dehydratase (HEM2)	Alad	20	36.0	6.3	263	P10518
8	Actin, alpha cardiac muscle 1 (ACTC)	Actc 1	38	41.8	5.2	606	P68033
9	Histone H2B type 1-B (H2B1B)	Hist1h2bb	27	13.8	10.3	67	Q64475
10	Peptidyl prolyl cistrans isomerase C (PPIC)	Ppic	14	22.8	7.0	172	P30412
11	2 Amino 3 ketobutyrate CoA ligase (KBL)	Goat	39	42.8	6.5	2133	O88986
12	Triosephosphate isomerase (TPIS)	Tip1	70	32.2	5.6	1581	P17751
13	Galectin 3 (LEG3)	Lgals3	29	27.6	8.5	500	P16110
14	Citrate synthase mitochondrial (CISY)	Cs	26	49.0	8.2	293	Q9CZU6
15	AP-5 complex subunit beta 1 (AP5B1)	Ap5b1	1	94.0	5.7	22	Q3TAP4
16	Elongation factor 1 alpha-1 (EF1A1)	Ef1a1	29	50.1	9.1	500	P10126

^a2-DE gel image spot number presented in Fig. 1
^bCommonly used protein name
^cGene symbol
^dPercentage of amino acid sequences for the identified protein
^eMW and pI = molecular weight and isoelectric point of the protein
^fScore = [−10 log (P)] and P is the absolute probability that the observed match between the experimental data and the database sequence is a random event, obtained with MASCOT for all proteins listed
^gSwiss-Prot primary protein accession number