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Table 3 Proteins separated by 2-DE electrophoresis and identified by MS in muscle tissue

From: Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice

Spota

Identification (Swiss-Prot)b

Genec

Sequencec coverage (%)

Spotd MW (KDa)

Spote pI

Scoref

Swissg Prot access #

1

Phosphoglucomutase-1 (PGM1)

Pgm1

45

61.7

6.1

816

Q9DOF7

2

Fatty acid-binding protein, heart (FABPH)

Fabp3

59

14.8

6.1

790

P11404

3

Fatty acid-binding protein, heart (FABPH)

Fabp3

66

14.8

6.1

533

P11404

4

Galectin-1 (LEG1)

Lgals1

31

15.2

5.3

135

P16045

5

Fumarate hydratase, mitochondrial (FUMH)

Fh

1

54.5

9.1

15

P97807

  1. a2-DE gel image spot number presented in Fig. 1
  2. bCommonly used protein name
  3. cGene symbol
  4. dPercentage of amino acid sequences for the identified protein
  5. eMW and pI = molecular weight and isoelectric point of the protein
  6. fScore = [−10 log (P)] and P is the absolute probability that the observed match between the experimental data and the database sequence is a random event, obtained with MASCOT for all proteins listed
  7. gSwiss-Prot primary protein accession number