Fig. 2

Relative levels of specific mRNA for ∆5D, ∆6D, Scd 1, and Scd 2 in the liver of the HiCHO and control groups. HiCHO high-carbohydrate (68%) diet-fed study group (n = 6); ST rats standard diet-fed controls (n = 6). Male Wistar rats weighing 151 ± 3 g were fed until achieved 250 g (21 days HiCHO vs. 25 days ST). Each gene was amplified together with a housekeeping gene β-actin (Actb; internal control). The expression was determined by RT-PCR. *p < 0.05. PCR products were separated on 1.5% agarose gel, stained with ethidium bromide, and analyzed by densitometry using the Quantity One 4.2.1. software. Obtained data were calculated to the mean value of the 200-bp band marker first and then normalized to β-actin (in the same sample). Shapiro-Wilk test was used for normality assessing. Student’s t test for unpaired samples; *p < 0.05