Fig. 4

The relative percentage of individual fatty acids in the liver of the HiCHO and control groups. HiCHO high-carbohydrate (68%) diet-fed study group (n = 6); ST rats standard diet-fed controls (n = 6). Male Wistar rats weighing 151 ± 3 g were fed until achieved 250 g (21 days HiCHO vs. 25 days ST). Fatty acid composition was determined using gas chromatography. Shapiro-Wilk test was used for normality assessing. Student’s t test for unpaired samples (data are presented as mean ± SD) was used to analyze the composition of 14:0, 16:0, 16:1 n-7, 17:0, 18:0, 18:1 n-9, 18:2 n-6, 18:3 n-6, 21:0, 22:0, 20:3 n-6, and 20:5 n-3, and Mann-Whitney U test (data are presented as median and range) was used to analyze the composition of 12:0, 20:4 n-6, and 22:6 n-3; *p < 0.05