Fig. 1

The validation of NGS results with real-time PCR—comparison of control and experimental group. Mean expression of ten genes: transthyretin (TTR), apolipoprotein C-III (APOC3), retinol-binding protein 4 (RBP4), ADAM metallopeptidase domain 19 (ADAM19), fatty acid desaturase 1 (FADS1), fatty acid desaturase 2 (FADS2), adenosine A1 receptor (ADORA1), calsequestrin 1 (CASQ1), mitogen-activated protein kinase 1 (MAPK1), Fas (TNFRSF6)-associated via death domain (FADD), and C-C motif chemokine ligand 4 (CCL4) determined using real-time PCR for 24 pigs from two diet treatments. Expression was normalized to the topoisomerase (DNA) II beta (TOP2B) gene as an endogenous control. Error bars represent standard error.*P value <0.05; **P value <0.01; ***P value <0.001, ns not significant