Fig. 1

Experimental design. Six-week-old C57BL/6J male mice were randomly assigned to three experimental groups (four or five animals per group). The mice were fed a standard diet and orally supplemented daily with MBC microbiota, LGG, or PBS (CTRL). After 15 days, the mice were shifted to HFD while continuing bacterial or PBS supplementation for 90 additional days. At the end of the experimental period, blood and epididymal WAT were collected. Faeces were sampled for gut microbiota analysis at the indicated time points: t0, t15, and t105. The experiment was replicated once, and the number of mice in each group for each of the two repetitions is indicated