Fig. 2

Leukocyte subpopulations in epididymal WAT. The effect of bacterial supplementation on the frequency of WAT leukocyte subpopulations was analysed by flow cytometry. The percentage of CD25⁺Foxp3⁺ Treg cells was calculated on T lymphocyte gate (CD4+, a), CD4⁺ and CD8⁺ cell subsets were calculated on lymphocyte gate (CD3⁺, b), whereas CD11b⁺ and F4/80⁺ cells were calculated on leukocyte gate (CD45⁺, c). Black columns: MBC-supplemented mice; grey columns: LGG-supplemented; white columns: CTRL. Each column represents the mean ± SD of nine mice. Means without a common letter significantly differ