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Table 3 Primers, locations, and functions

From: High-fat diet modifies expression of hepatic cellular senescence gene p16(INK4a) through chromatin modifications in adult male rats

Gene

Sequence

Function

Ensembl ID

p16(INK4a)

Forward (p16(INK4a) + 12F)

5′-TGCAGATAGACTAGCCAGGGGA-3′

Reverse (p16(INK4a) + 76R)

5′-CTTCCAGCAGTGCCCGCA-3′

mRNA

ENSRNOT00000066011

p16(INK4a)

Forward (p16(INK4a) − 61F)

5′-ACTGGGCGGGCACTGAATCTC-3′

Reverse (p16(INK4a) + 101R)

5′-TCCGGGGCGTTTGGTGAAG-3′

ChIP promoter

 

p16(INK4a)

Forward (p16(INK4a) + 5829F)

5′-CGGGTCACCGACAGGCATAA-3′

Reverse (p16(INK4a) + 5889R)

5′-TTGGACCACCCATGCTCACC-3′

ChIP coding and transcription rate

 

p16(INK4a)

Forward (p16(INK4a) + 7947F)

5′-TCCGAGTCCCCATCTGTGACTGT-3′

Reverse (p16(INK4a) + 8016R)

5′-TGGAGGATGGATTGGAGCACC-3′

ChIP downstream

 

p21(Cip1)

Forward (p21(Cip1) + 5100F)

5′-CCGAGAACGGTGGAACTTTGAC-3′

Reverse (p21(Cip1) + 5171R)

5′-GAACACGCTCCCAGACGTAGTTG-3′

mRNA and ChIP

ENSRNOT00000000628

p21(Cip1)

Forward (p21(Cip1) − 320F)

5′-GACAGACCTGTAAACGCTCAAC-3′

Reverse (p21(Cip1) − 233R)

5′-ACACCTGCTTGTCACGCA-3′

ChIP promoter

 

p21(Cip1)

Forward (p21(Cip1) − 5200F)

5′-GGCTCACTTACACTTCCCAG-3′

Reverse (p21(Cip1) − 5125R)

5′-GGTTTCATTGGCTGGAGC-3′

ChIP upstream

 

p21(Cip1)

Forward (p21(Cip1) + 6388F)

5′-AAAACGGAGGCAGACCAG-3′

Reverse (p21(Cip1) + 6450R)

5′-TCCCGCAGTATCTTGCCT-3′

Transcription rate

 

L7a

Forward (L7a + 67F)

5′-GAGGCCAAAAAGGTGGTCAAT-3′

Reverse (L7a + 127R)

5′-CCTGCCCAATGCCGAAGTTCT-3′

mRNA

ENSRNOT00000006754