Fig. 5
Effect of DHA on the microRNA-16-1 expression. 5 × 105 HCT-116 cells per well were treated with 100 and 150 μM/L DHA for 48 h, after which microRNA-16-1 expression levels were measured by real-time RT-PCR method. Parallel Real time RT-PCR with U6 snRNA specific primers was performed to normalize the equal loading. The results were presented as mean ± SD. *P < 0.05 vs. untreated control cells (C)