Table 2 Primers for real-time qPCR: primers for mRNA
No. | Primer’s name | Forward sequence | Reverse sequence | Annealing time and temperature |
|---|---|---|---|---|
1 | otud4 | CCACCTCCTGCAGAACAAAAG | GGGTCTTGGTAAAGGCTCATT | 15 s/60 °C |
2 | cx43 (gja1) | TGCTGCGAACCTACATCATC | CGATGACGTTCAAGGCAAGA | 15 s/60 °C |
3 | sod2 | GTCACCGAGGAGAAGTACCA | CTGGTTAGGACAGGCAACAA | 15 s/60 °C |
4 | sod1 | GATTCCACGTCCACGAGTTT | CCCGAGAGTGAGATCACAGA | 15 s/60 °C |
5 | tgfb2 | AGTACTACGCCAAGGAGGTT | TAGGCGGGATGGCATTTTCC | 15 s/60 °C |
6 | myf5 | GGAGACGCCTGAAGAAAGTC | CCGGCAGGCTGTAGTAATTC | 15 s/60 °C |
7 | bdnf | CCCCATGAAAGAAGCAAACG | TACAAGTCCGCGTCCTTACT | 15 s/60 °C |
8 | gapdh | GTTTGTGATGGGCGTGAACC | GTCTTCTGGGTGGCAGTGAT | 15 s/60 °C |