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Fig. 5 | Genes & Nutrition

Fig. 5

From: Optimal vitamin D spurs serotonin: 1,25-dihydroxyvitamin D represses serotonin reuptake transport (SERT) and degradation (MAO-A) gene expression in cultured rat serotonergic neuronal cell lines

Fig. 5

Effect of 1,25D on total 5-HT levels in rat serotonergic neuron cell culture medium. a A dose-response experiment was performed in serotonergic rat raphe RN46A-B14 cells treated with the indicated concentrations of 1,25D or ethanol (EtOH) vehicle. A 200 μL sample of medium was removed from each plate after 72 h of treatment and 5-HT quantitated by ELISA as detailed in the “Methods” section. Values are the average of three biological replicates (n = 3) ± SD, with duplicate or triplicate samples averaged in each group. b A time-course experiment was carried out in RN46A-B14 cells treated with the optimal concentration of 1,25D (10 nM) for 0, 24, 48, and 72 h followed by removal of a 200 μL sample of medium from each plate and serotonin assayed by 5-HT ELISA. Values are the average of three biological replicates (n = 3) ± SD, with duplicate or triplicate sample groups averaged in each case

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Genes & Nutrition

ISSN: 1865-3499

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