Table 1 Characteristics of primers used for real-time quantitative PCR analysis
Gene | NCBI GenBank | Primersa | Primer sequence(5′ → 3′) | Amplicon (bp)b |
|---|---|---|---|---|
LPL | NM_214286.1 | F.654 | ATTGCAGGAAGTCTGACCA | 124 |
R.777 | CGTCTACAAAATCTGCGTC | |||
CD36 | DQ192230.1 | F.433 | GGACTCATTGCTGGTGCTGT | 169 |
R.601 | GTCTGTAAACTTCCGTGCCTGT | |||
ACSL3 | NM_001143698.1 | F.470 | ACCCTGGATGTGATACGCTA | 150 |
R.619 | AGTTCCCAAGAATAACCTTTT | |||
FABP3 | AY569332.1 | F.157 | CTGGGAGTGGAGTTTGATGAGAC | 164 |
R.320 | CCATGGGTGAGTGTCAGGAT | |||
ACACA | NM_001114269.1 | F.3638 | ACATCCCCACGCTAAACA | 186 |
R.3823 | AGCCCATCACTTCATCAAAG | |||
FASN | NM_001099930.1 | F.1884 | GCTTGTCCTGGGAAGAGTGTA | 115 |
R.1998 | AGGAACTCGGACATAGCGG | |||
SCD | NM_213781.1 | F.785 | TGACCTAAAAGCCGAGAA | 164 |
R.948 | GCACGATGGCGTAACGAAGA | |||
GPAM | XM_001927875.1 | F.955 | ACTATCTCCTGCTCACTTTCA | 146 |
R.1100 | CGTCTCATCTAGCCTCCGTC | |||
AGPAT1 | EU282358.1 | F.170 | CCTTCTACAACGGCTGGAT | 174 |
R.343 | GCTGTGAGGGAGGGAAGTGG | |||
AGPAT6 | FJ439669.1 | F.56 | CTGGGCATCTCCCTGACTGT | 198 |
R.253 | GATTCCATTGGTGTAGGGCTTG | |||
DGAT1 | AY116586.1 | F.6569 | TGGACTACTCACGCATCAT | 176 |
R.6744 | GTGGAAGAGCCAGTAGAAGAA | |||
LPIN1 | NM_001130734.1 | F.2084 | CACATTTTGCCCACCCTT | 164 |
R.2247 | GTGCCACGCTCGTTGACC | |||
LPIN2 | NM_001141987.1 | F.2026 | CCTATGGAACTGGAACGA | 141 |
R.2166 | TTGATGGAGTGGTAGAGCTTGG | |||
PLIN2 | NM_214200.2 | F.1183 | CTCCTCAGTTCCAGCAAG | 113 |
R.1295 | GGATAAAAGGGACCTACCAG | |||
SREBP1 | NM_214157.1 | F.984 | AGCGGACGGCTCACAATG | 121 |
R.1104 | CGCAAGACGGCGGATTTA | |||
INSIG1 | NM_001244521.1 | F.509 | TGTCGTGGGCTTGCTCTA | 123 |
R.631 | GCACTGGCGTGGTTGATG | |||
SCAP | AY705448.2 | F.1089 | GCGGTGAGATTTTCCCCTAC | 185 |
R.1273 | GCCAATGAGGATGATGCC | |||
PPARα | DQ437887.1 | F.323 | CAGCGTGGCACTGAACATC | 144 |
R.466 | CTCCGATCACATTTGTCATAGAC | |||
PPARγ | NM_214379.1 | F.1170 | AGCCCTTTGGTGACTT | 213 |
R.1382 | AGGACTCTGGGTGGTT |