Skip to main content

Table 1 Primer sequence and the annealing temperature used for methylation-specific PCR

From: The effects of dietary polyunsaturated fatty acids on miR-126 promoter DNA methylation status and VEGF protein expression in the colorectal cancer cells

Gene

Forward primer

Reverse primer

Annealing T ( °C)

miR-126

U: 5′-GTGGTGGTGGTGTGTGTGTGTTT-3′

5′-CTCAACCCAACCCAAACAACAACCA-3′

60

M: 5′-GCGGCGCGTGCGCGTTT-3′

5′-CCAACCCGAACGACGACCG-3′