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Table 1 Primer sequence and the annealing temperature used for methylation-specific PCR

From: The effects of dietary polyunsaturated fatty acids on miR-126 promoter DNA methylation status and VEGF protein expression in the colorectal cancer cells

Gene Forward primer Reverse primer Annealing T ( °C)
miR-126 U: 5′-GTGGTGGTGGTGTGTGTGTGTTT-3′ 5′-CTCAACCCAACCCAAACAACAACCA-3′ 60
M: 5′-GCGGCGCGTGCGCGTTT-3′ 5′-CCAACCCGAACGACGACCG-3′