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Fig. 6 | Genes & Nutrition

Fig. 6

From: The peptide transporter 1a of the zebrafish Danio rerio, an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis

Fig. 6

Expression analysis by RT-PCR on pept1a (slc15a1a) mRNA in adult zebrafish tissues. a RT-PCR assay on cDNA templates from total RNA extracted from various tissues; a PCR product of ~ 350 bp related to pept1a (slc15a1a) mRNA is present in samples from the intestine and ovary, while it is absent in the eye, gills, kidney, spleen, liver, pancreas, and brain; using the same cDNA templates, a PCR product of ~ 440 bp related to the actb mRNA is present in all tissue samples; L: 1 Kb Plus DNA ladder (Thermo Fisher Scientific). b Comparative table of pept1a (slc15a1a) vs. pept1b (slc15a1b) mRNA presence in the different zebrafish tissues analyzed. pept1b (slc15a1b) tissue expression data are from [14]. +, positive detection; n.d., not detected; n.i., not investigated

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