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Table 2 Kinetic parameters of the inwardly directed transport of Gly-Gln via the zebrafish PepT1a (Slc15a1a) and zebrafish PepT1b (Slc15a1b) measured in two-electrode voltage clamp experiments

From: The peptide transporter 1a of the zebrafish Danio rerio, an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis

 

− 60 mV

− 120 mV

 

pH

Neutral form (%)

K0.5 (mmol/L)

Imax (nA)

Imax/K0.5 (nA/mmol/L)

K0.5 (mmol/L)

Imax (nA)

Imax/K0.5 (nA/mmol/L)

Oocytes/batches (n/N)

PepT1a

 6.5

98.4

0.24 ± 0.07

− 75.76 ± 6.04

316.37

0.45 ± 0.19

− 157.39 ± 21.48

350.81

9/3

 7.6

83.0

6.92 ± 2.34

− 169.57 ± 43.75

24.51

3.61 ± 0.73

− 378.82 ± 53.08

105.02

14/3

PepT1b

 6.5

98.4

0.13 ± 0.02

− 195.73 ± 8.89

1535.32

0.13 ± 0.02

− 396.24 ± 22.21

3032.16

7/1

 7.6

83.0

2.22 ± 1.04

566.16 ± 212.44

254.54

1.01 ± 0.35

1142.30 ± 285.34

1129.67

7/1

  1. Kinetic parameters (K0.5, Imax, and Imax/K0.5) were calculated on Xenopus laevis oocytes voltage clamped at − 60 mV and at − 120 mV and perfused with Gly-Gln in sodium chloride buffer solutions at pH 6.5 and 7.6. Values are expressed as means ± SEM of n oocytes (each oocyte represents an independent observation). Kinetic parameters were calculated by least-square fit to the logistic equation (Fig. 2). Imax/K0.5, transport efficiency
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Genes & Nutrition

ISSN: 1865-3499

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