Fig. 1
Cerulein-induced translocation of the E subunit of vATPase to the membrane. Cells were stimulated with cerulein (10−8 M) for the indicated time periods. a Levels of GPR120, GPR40, and vATPase E subunit in total cell extracts were determined by western blot analysis. Actin was used as a loading control. b Levels of vATPase E subunit in cytosolic and membrane fractions were determined by western blot analysis. Aldolase A and Na+/K+-ATPase were used as markers of cytosolic and membrane fractions, respectively