Fig. 9

Schematic pathways for inhibitory effect of DHA on cerulein-induced activation of zymogen in AR42J cells. Cerulein induces the translocation of the cytosolic V1 domain of vATPase to the membrane, which may assemble with the V0 domain to activate vATPase in pancreatic acinar cells. vATPase activation may result in zymogen activation in pancreatic acinar cells. DHA binds to GPR120 and GPR40 which increases the level of Parkin in membrane. Parkin may induce the ubiquitination and degradation of membrane vATPase. Thus, DHA suppresses vATPase-mediated zymogen induced by cerulean. In summary, DHA inhibits zymogen activation, via GPR120 and GPR40, in pancreatic acinar AR42J cells stimulated with cerulein. Red arrows represent the effect of DHA. Black arrows mean the effect of cerulein stimulation