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Table 2 Overview of the validation criteria for candidate intake biomarkers for banana and tropical fruitsa

From: Biomarkers of intake for tropical fruits

Food item

Metabolites

Biofluid

Q1

Q2

Q3a

Q3b

Q4

Q5

Q6

Q7

Q8

Banana

5-Hydroxyindole acetic acid

Plasma

Y

U

Y

U

U

U

Y

Y

U

Banana

5-Hydroxyindole acetic acid

Urine

Y

U

Y

U

U

U

Y

Y

U

Banana

3-Methoxytyramine sulfate

Plasma

Y

U

U

U

Y

U

U

U

U

Banana

Dopamine sulfate

Plasma

Y

U

Y

U

Y

U

Y

Y

U

Banana

Dopamine sulfate

Urine

Y

Y*

Y

U

U

U

Y

Y

U

Banana

Methoxyeugenol glucuronide

Urine

Y

Y*

Y

U

U

U

U

U

U

Banana

Salsolinol sulfate

Urine

Y

Y*

Y

U

U

U

U

Y

U

Banana

6-OH-MTβC sulfate

Urine

Y

Y*

U

U

U

U

U

U

U

Banana

Methoxyeugenol glucuronide + Dopamine sulfate

Urine

Y

Y*

Y

U

Y

Y

U

U

U

Banana

Methoxyeugenol-glucuronide + Dopamine sulfate + Salsolinol sulfate

Urine

Y

Y*

Y

U

Y

Y

U

U

U

Banana

Methoxyeugenol glucuronide + Dopamine sulfate + 6-OH-MTβC sulfate + 2-isopropylmalic acid

Urine

Y

Y*

Y

U

Y

Y

U

U

U

Banana

Methoxyeugenol glucuronide + Dopamine sulfate + Salsolinol sulfate + Xanthurenic acid + 6-OH-MTβC sulfate

Urine

Y

Y*

Y

U

Y

Y

U

U

U

Avocado

Perseitol

Urine

Y

U

Y*

U

U

U

U

U

U

Avocado

Mannoheptulose

Urine

Y

U

Y*

U

U

U

U

U

U

Watermelon

Citrulline

Plasma

Y

U

U

U

U

U

U

U

U

Watermelon

Citrulline

Urine

Y

U

U

U

U

U

U

U

U

  1. aQuestions related to each of the validation criteria: Q1. Is the marker compound plausible as a specific BFI for the food or food group (chemical/biological plausibility)?, Q2. Is there a dose-response relationship at relevant intake levels of the targeted food (quantitative aspect)?, Q3. Is the biomarkers kinetics described adequately to make a wise choice of sample type, frequency and time window (time-response) [a single-meal time-response relationship, b: repeated intakes have]?, Q4. Has the marker been shown to be robust after intake of complex meals reflecting dietary habits of the targeted population (robustness)?, Q5. Has the marker been shown to compare well with other markers or questionnaire data for the same food/food group (reliability)?, Q6. Is the marker chemically and biologically stable during bio specimen collection and storage, making measurements reliable and feasible (stability)?, Q7. Are analytical variability (CV %), accuracy, sensitivity, and specificity known as adequate for at least one reported analytical method (analytical performance)?, Q8. Has the analysis been successfully reproduced in another laboratory (reproducibility)?
  2. Y yes the criterion is fulfilled, Y* the criterion is partially fulfilled but requires further investigation, N no the principle has not been fulfilled after investigation, U unknown; further data is required to determine the validation of the criterion, 6-OH-MTβC sulfate 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-β-carboline sulfate
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Genes & Nutrition

ISSN: 1865-3499

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