Fig. 1

Effects of antibiotics on body weight gain in the high-fat diet-fed mice. Three to 4-week-old C57BL/6J male mice were fed a high-fat diet to induce obesity (DIO group) for 16 weeks, with a normal-fat diet as control (NC group). Meanwhile, ampicillin (1 g/L) and neomycin (0.5 g/L) were delivered via drinking water to mice fed the high-fat diet (DIO-AB group) and the normal-fat diet (NC-AB group). Antibiotic use lessened the weight gain (a, b) with no changes in energy intake (c), and reduced adipocyte size (d, e). n = 10 in each group. Data are means ± SD. *Compared to the NC group, P < 0.05; ^#compared to the DIO group, P < 0.05