Alteration of gut microbiota affects expression of adiponectin and resistin through modifying DNA methylation in high-fat diet-induced obese mice

Table 3 Quantitative methylation analysis of the adipokine promoters

Adiponectin	CG2	CG3	CG4	CG5	CG6	Average
NC	79.6 ± 2.7	72.4 ± 4.4	82.8 ± 1.0	77.6 ± 4.1	69.8 ± 2.4	76.4 ± 5.3
NC-AB	81.2 ± 4.3	75.4 ± 4.8	83.4 ± 2.2	76.7 ± 1.1	67.0 ± 5.7	76.7 ± 6.3
DIO	83.9 ± 4.4*	80.4 ± 2.9*	90.7 ± 2.8*	84.0 ± 1.5*	75.9 ± 2.8*	83.0 ± 5.4
DIO-AB	79.5 ± 4.3^#	78.2 ± 3.2*	86.2 ± 3.9*^#	79.5 ± 4.3^#	69.0 ± 2.6^#	78.5 ± 6.2
Resistin	CG2	CG3	CG5	CG6	CG7	CG8	CG9	CG10	CG11	CG12	CG13	CG14	CG15	CG16	Average
NC	79.3 ± 7.3	90.7 ± 3.3	79.0 ± 4.8	86.9 ± 3.4	68.2 ± 4.6	80.2 ± 2.9	72.6 ± 4.5	74.3 ± 4.0	65.5 ± 4.2	56.5 ± 2.0	73.3 ± 6.8	78.4 ± 8.7	74.8 ± 4.7	76.2 ± 9.0	75.4 ± 8.5
NC-AB	81.6 ± 5.9	90.2 ± 4.6	81.6 ± 3.9	85.3 ± 3.4	71.1 ± 5.1	76.7 ± 4.0	76.8 ± 5.3	77.5 ± 2.1	67.7 ± 3.8	56.4 ± 2.0	80.4 ± 7.8	80.4 ± 13.4	77.3 ± 5.2	80.7 ± 8.5	77.4 ± 8.2
DIO	91.1 ± 3.8*	93.4 ± 4.9	87.8 ± 5.4*	86.8 ± 5.5	75.7 ± 6.2*	80.9 ± 7.5	79.0 ± 2.0*	82.9 ± 5.5*	69.5 ± 6.6	55.9 ± 1.3	85.1 ± 6.4*	88.8 ± 6.3*	83.5 ± 8.4*	78.5 ± 6.0	81.4 ± 9.7
DIO-AB	84.6 ± 6.9	91.3 ± 4.6	80.4 ± 6.9^#	85.9 ± 1.2	68.0 ± 5.2^#	79.5 ± 6.5	75.0 ± 2.8^#	74.5 ± 5.6^#	72.3 ± 6.1	58.4 ± 3.1	79.7 ± 7.5	87.3 ± 5.9	75.7 ± 10.2	74.1 ± 4.9	77.6 ± 8.5

Genomic DNA isolated from epididymal fat was analyzed for the methylation of CpG sites at the indicated positions in the promoters of adiponectin and resistin genes spanning nucleotides -1162 to -494 and -1450 to -113 respectively, relative to the TSS. The methylation fraction was calculated from the amplitude of cytosine and thymine within each CpG dinucleotide, C/(C+T)*100. The result for each CpG site is represented as the means ± SD. n = 10 in each group
*Compared to the NC group, P < 0.05
^#Compared to the DIO group, P < 0.05

ISSN: 1865-3499