Fig. 1

Body weight (a), Oil red O (ORO) staining in the liver (b), and lipid contents in longissimus dorsi (LD) muscle tissues (c) of sham-operated (Sham) and castrated (Cast) male rats fed an ND or high fat diet (HFD). a Values are means + SE (n = 7–8 rats per group). *P < 0.05 and **P < 0.01, significant differences between the Sham and Cast groups. b Scoring of hepatic lipid droplet accumulation (0 = none, 5 = highest). Black bar, 100 μm. b, c Values are means + SE (n = 7–8 per group). ^a–cMeans with different superscripts differ (P < 0.05)