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Fig. 1 | Genes & Nutrition

Fig. 1

From: DNA methylation during human adipogenesis and the impact of fructose

Fig. 1

Overview of the integration analysis of the three available omics data: CpG methylation levels, gene expression, and protein abundances. The steps referring to single-omics analysis are done prior to integration and are depicted in boxes of different colors: green for gene expression, blue for methylation, and orange for proteomics. On the left of the schema, moderated F-statistics identify the differentially expressed genes (DEGs). The pipeline for methylation analysis is in the middle of the panel: differentially methylated positions (DMPs) were found, then DMPs in the same genomic region were assembled identifying differentially methylated regions (DMRs). The proteomics analysis (right) shows differentially expressed proteins (DEPs) identified with moderated t-statistics. Gray boxes at the bottom of the schema represent the steps of multi-omics integration. First, concordant changes among the epigenomic and transcriptomic levels were studied. Then, associations were made with differentially expressed proteins to find genes changing in methylation, gene expression, and protein levels

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