Fig. 1
Western blots of B0AT1, ASCT2, LAT2, and LAT4 protein. Blots are representative of a total of 188 western blots evaluated for this study. Blots for B0AT1, LAT2, and LAT4 were conventional western blots where specific signals were quantified relative to RPL19 as loading control. For ASCT2, stain-free technology was used and ASCT2 protein intensity was quantified relative to total protein. Tissues were grouped per animal on blots for B0AT1 and LAT4, whereas tissues from the three feeding groups were compared on blots for LAT2 and ASCT2. The left lane in each blot shows the molecular weight marker. IRC, inter-run calibrator; DUO, duodenum; PJ, proximal jejunum; MJ, middle jejunum; IL, ileum; CAE, caecum; PC, proximal colon; DC, distal colon