Table 1 Primer and probe sequences for the Met transporters and reference genes
Gene | Accession number | Primer | Sequence | Probe sequence |
|---|---|---|---|---|
B0AT1 (SLC6A19) | XM_003359855.4 | Fwd | CTTCATCTTCACCCTGAACTC | CCCCTGCTCATCATCGCCTTCTTCGAGATGT |
Rev | GATGTCGCTGTTGAACCTG | |||
ATB0+ (SLC6A14) | NM_001348402.1 | Fwd | CTGTGGCTTGGGGTGGTTTA | CCAACTCCCAGGTGGGCCAT |
Rev | AACCAAGCAGCAACCCAAAG | |||
rBAT (SLC3A1) | NM_001123042.1 | Fwd | CAATGCAGTGGGACAACAG | TCCAAAAGACCCAGCCCAAATCAGCA |
Rev | GGCGTGAAGCAAACTTAATTC | |||
ASCT2 (SLC1A5) | XM_003127238.4 | Fwd | CGATTCGTTCCTGGATCTTG | CTCCAACCTGGTGTCTGCAGCCTT |
Rev | TAGGACGTCGCGTATGAG | |||
IMINO (SLC6A20) | XM_003358406.4 | Fwd | TCGTGTCCCTCATCAACAG | ACCTCCATCTTTGCCAGTGTCGTCACCTT |
Rev | AGGAAGCCATCTTCAAGGTC | |||
LAT4 (SLC43A2) | XM_003358191.3 | Fwd | CAGATCCAGAAGATCACCAAC | TGACCTGCCTCATTCCCAACCTGC |
Rev | TGAAGGAGAGAATCTGTAGGG | |||
y+LAT1 (SLC7A7) | NM_001110421.1 | Fwd | CTCTGCTGTTCAATGGTCTC | GGCGCTGATCTACTTGTGCGTGGA |
Rev | ATAGAGCTGACCCACGATAG | |||
LAT2 (SLC7A8) | XM_003128550.5 | Fwd | ACTACCTCTTCTATGGCATCAC | CGGACAGATAGTTCTTCGCTGGAAGAAGCCTAA |
Rev | GCAAGTAGATGATGGGGAACAG | |||
SNAT2 (SLC38A2) | XM_003126626.5 | Fwd | TTCATTCTTCCATCTGCCTTC | GTTAAGTGGCATAGGGGTGATGACCGGA |
Rev | GGGCATTGTGTACCCAATC | |||
ACTB | XM_021086047.1 | Fwd | GACATCAAGGAGAAGCTGTG | CTGGACTTCGAGCAGGAGATGGCC |
Rev | CGTTGCCGATGGTGATG | |||
GAPDH | XM_021091114.1 | Fwd | CAAGAAGGTGGTGAAGCAG | TGAGGACCAGGTTGTGTCCTGTGACTTCAA |
Rev | GCATCAAAAGTGGAAGAGTG | |||
YWHAZ | XM_005662949.2 | Fwd | AAGAGTCATACAAAGACAGCAC | ATCGGATACCCAAGGAGATGAAGCTGAA |
Rev | ATTTTCCCCTCCTTCTCCTG |