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Table 5 Relative overall ranking

From: Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

 

Ranking

BestKeeper

ΔCq

NormFinder

RefFinder

Overall

Most stable

1

Hprt

Rpl19

Rps29

Rpl19

Rpl19

2

Rpl19

Actb

Rpl27

Rps29

Rps29

Least stable

1

Gapdh

Rplp0

Gapdh

Gapdh

Gapdh

2

Actb

Ppia

Hprt

Ppia

Ppia

  1. The two most stable and two least stable RG from BestKeeper, comparative ΔCq, NormFinder, and RefFinder were combined to provide an overall ranking of PF and ID experimental groups in all tissues. Overall ranking was determined by quantifying the most stable or least stable genes of all programs
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Genes & Nutrition

ISSN: 1865-3499

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