Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

Table 6 Relative overall ranking for each tissue

Gastrocnemius	Ranking	BestKeeper	ΔCq	NormFinder	RefFinder	Overall
Most stable	1	Gapdh	Ppia	Ppia	Ppia	Ppia
Most stable	2	Ppia	Rpl22	Tbp	Gapdh	Gapdh
Heart	Ranking	BestKeeper	ΔCq	NormFinder	RefFinder	Overall
Most stable	1	Hprt	Rps29	Rps29	Hprt	Rps29
Most stable	2	Tbp	Rpl19	Rpl19	Rps29	Rpl19/Hprt*
Kidney	Ranking	BestKeeper	ΔCq	NormFinder	RefFinder	Overall
Most stable	1	Gapdh	Ppia	Tbp	Rps29	Rps29
Most stable	2	36b4	36b4	Rps29	Rpl22	36b4
Liver	Ranking	BestKeeper	ΔCq	NormFinder	RefFinder	Overall
Most stable	1	Tbp	Rpl27	Rpl27	Rpl27	Rpl27
Most stable	2	Rpl27	Rpl19	Rpl19	Rpl19	Rpl19
Lung	Ranking	BestKeeper	ΔCq	NormFinder	RefFinder	Overall
Most stable	1	Gapdh	36b4	Tbp	36b4	36b4*
Most stable	2	Hprt	Tbp	36b4	Tbp	Tbp*
Spleen	Ranking	BestKeeper	ΔCq	NormFinder	RefFinder	Overall
Most stable	1	36b4	Rps29	Ppia	Ppia	Gapdh*
	2	Gapdh	Rpl22	Gapdh	Rps29	Ppia*
						Rps29*

The two most stable RG from BestKeeper, comparative ΔCq, NormFinder, and RefFinder were combined to provide an overall ranking of PF and ID experimental groups in gastrocnemius, heart, kidney, liver, lung, and spleen. Overall ranking for each tissue was determined by quantifying the most stable genes of all programs. *Indicates equal stability ranking

ISSN: 1865-3499