Table 8 Primer sequences for reference gene analysis by qPCR
Gene symbol | Forward primer | Reverse primer |
|---|---|---|
Actb | 5′ CAT CGT GGG CCG CCC TA | 5′ CGC CCA CGG AGG AGT CCT TCT G |
Gapdh | 5′ GAG GTG ACC GCA TCT TCT TG | 5′ CCG ACC TTC ACC ATC TTG TC |
Hprt | 5′ GCC GAC CGG TTC TGT CAT | 5′ CAT AAC CTG GTT CAT CAT CAC TAA TCA |
Ppia | 5′ GGT CTT TGG GAA GGT GAA AGA A | 5′ GCC ATT CCT GGA CCC AAA A |
Rpl19 | 5′ CGT CCT CCG CTG TGG TAA A | 5′ TGG CGA TTT CGT TGG TTT |
Rpl22 | 5' CAC CCT GTA GAA GAT GGA ATC ATG | 5' TTC CCG TTC ACC TTG ATC CT |
Rpl27 | 5′ GCA AAG CCG TCA TCG TAA AGA | 5′ CTG GGA TAG CGG TCA ATT CC |
Rplp0 | 5′ CAC CTT CCC ACT GGC TGA A | 5′ TCC TCC GAC TCT TCC TTT GC |
Rps29 | 5′ GCC AGG GTT CTC GCT CTT G | 5′ GGC ACA TGT TCA GCC CGT AT |
Tbp | 5′ TGC CAG AAA TGC TGA ATA TAA TCC | 5′ GTT CGT GGC TCT CTT ATT CTC ATG |
Tfrc | 5′ TCG GCT ACC TGG GCT ATT GT | 5′ CCG CCT CTT CCG CTT CA |