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Fig. 3 | Genes & Nutrition

Fig. 3

From: Programmed death-ligand 1 signaling and expression are reversible by lycopene via PI3K/AKT and Raf/MEK/ERK pathways in tongue squamous cell carcinoma

Fig. 3

Knockdown of PD-L1 inhibited SCC9 cell proliferation, migration, and invasion and promoted apoptosis, while lycopene aggravated the effect. A The knockdown efficiency of si-CD274. There are two bands of PD-L1 up 65 ~ 70 KD, down 45 ~ 56 KD, according to instructions of the supplier, the down band is chosen to count knockdown efficiency at 75%. B CCK-8 assay was used to measure SCC9 cell proliferation. C and D Transwell assay was carried out to measure SCC9 cells migratory and invasive ability. E TUNEL was used to detect SCC9 cells apoptosis. *P < 0.05 vs NC, #P < 0.05 vs si-CD274. NC means negative control; BC means blank control. CD274 also known as PD-L1

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