Fig. 6

Effects of CD274 overexpression, LY294002, and lycopene on PI3K/AKT and Raf/MEK/ERK pathways. A Western bolt was performed to analyze PD-L1 and downstream key molecules p-IGF-1R, p-Akt1 (Ser473), p-Akt1 (Thr308), p-c-Raf, p-MEK1, p-ERK, and p-GSK-3β (Ser9) expressions. According to manufacturer’s instructions, there were two bands of PD-L1, p-ERK owing to post-translation modification. B Quantitative statistical analysis of A. *P < 0.05 vs NC, # P < 0.05 vs CD274. NC means negative control