Fig. 5
Animal model observations from the application of genistein. Male SD rats (3 to 4 weeks old) were divided into 4 groups (n = 6 per group). The hair on the back of the rat was removed 2 to 3 days before the experiments. The back of each animal was marked with India ink, and the marked area was divided into 4 squares (0.3 × 0.3 cm2 each); these squares were treated with 10 μL of each concentration (0.1% and 1%) of genistein solution in water, and one square was treated with water as a control area. The rats were pretreated with genistein once before they were exposed to UVB radiation (50 mJ/cm2); subsequently, skin wrinkling was investigated (magnification: 10×)